羊毛品质性状相关基因CCNY的验证和分析

doi:10.11843/j.issn.0366-6964.2017.08.003

Abstract

Abstract:

The aim of this study was to verify whether CCNY gene was associated with sheep wool production and quality traits, and to explore its possible mechanism. Seven hundred forty-four Chinese Merino sheep (Junken type) were used in the present study. SNPs were identified by Sanger sequencing of the pooled genomic DNA samples, and genotyped by MALDI-TOF-MS. Real-time RT-PCR was used to analyze the gene expression, and immunofluorescence staining was used to localize the protein. CCK-8 method was used to detect cell proliferation. Luciferase reporter gene assay was used to assess the activity of Wnt/β-catenin signaling pathway. The results showed that a total of 3 SNPs(CCNYSNP1, CCNYSNP2, CCNYSNP3) were identified in CCNY gene in Chinese Merino sheep. Association analysis displayed that the 3 identified SNPs and their haplotypes were significantly associated with wool fiber diameter (P<0.05). Tissue expression analysis showed that CCNY gene was highly expressed in testicle and lung, and moderately expressed in several tissues including skin. Immunofluorescence staining showed that CCNY was localized in inner root sheath and hair matrix of hair follicle. CCK-8 assay results showed that CCNY overexpression significantly promoted proliferation of HaCaT and SFF cells(P<0.05). Reporter gene analysis showed that CCNY overexpression significantly increased the activity of Wnt/β-catenin signaling pathway(P<0.01). These data suggest that CCNY is a crucial factor governing fiber diameter trait, and 3 identified CCNY SNPs might be used in molecular marker-assisted selection for fine wool sheep breeding.

CLC Number:

S826.2

ZHANG Wen-jian, MA Guang-wei, ZHANG Xiao-fei, CHU Yan-kai, LIU Jing, YANG Hua, YAN Xiao-hong, LI Yu-mao, LI Hui, WANG Ning. Verification and Analysis of Wool Quality Traits-associated CCNY Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1389-1400.

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Verification and Analysis of Wool Quality Traits-associated CCNY Gene

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